Shakers

Getting started with cell culture can seem like an overwhelming step: cells are sensitive to shear stress, have a high risk of contamination and are very demanding concerning the right choice of process parameters. Check our blog post for all you need to start shaking or running your bioreactor with mammalian cells, insect cells and plant cells.

What is one of the worst things that could go wrong in your laboratory? Ask anyone working to a deadline in a bioprocess laboratory and a likely answer would be contamination of their cultures. Contamination can be hard to trace back to a source and difficult to remove if it is present in your working environment. Fortunately, there are a few simple steps you can follow to minimise the chances of this happening to you.

Cleaning an incubator shaker sounds trivial but is critical for successful cultivation. The good news is that doing the right thing is simple, effective and can easily be integrated into the routine management of a biosciences laboratory.  

Mammalian cell culture bioprocesses are increasingly gaining importance in the biopharmaceutical industry. Due to the high demand of product quality, the incubated shaker as a production unit can become a limiting factor after a certain point. Moreover, since real-time monitoring and control is not possible in a shaker, eventual process transfer and scaling-up are indispensable measures.

The shake flask is the standard in many laboratories for cultivation of cells and microorganisms. Thereby achieving high biomass concentrations remains a constant challenge.