Bioreactors for continuous cultivation with perfusion

Perfusion is a continuous culturing method in which cells are either retained in the bioreactor or fed back into it. The harvested medium thus contains no cells, resulting in higher cell concentrations and product yields in the reactor while still reducing the working volume. This also prevents the risk of cell washout due to excessively high dilution rates (D).

Additional pumps and scales provide more feeding options

The standard features of integrated pumps are often insufficient for carrying out perfusion bioprocesses. Connecting additional, external pumps requires a bioreactor with additional analog inputs and outputs. To ensure that the external pump can communicate with the bioreactor, it helps to order the pump directly from the bioreactor manufacturer. Also, connecting one or more scales makes gravimetric feeding an option for precise nutrient addition over long periods of time.

A feeding profile for optimizing the introduction of nutrients

Regularly feeding additional nutrient medium keeps cells well supplied throughout the long perfusion process, increasing yields significantly and ensuring product quality. The pump for adding medium can either be controlled from the bioreactor directly or using a speific bioprocess software. A major advantage of the latter is that it can save individual feeding profiles and to use them again. Plus, bioprocess software can use parameters such as cell concentration to control the process of pumping medium in and out.

Freely configurable pumps save money

The advantage of being able to freely select the operating mode for the bioreactor's integrated pumps is that the pumps can then be reconfigured quickly and easily. If using CO2 to regulate the pH of a cell culture, operators could simply press a button to convert the acid pump to an analog feed and/or harvest pump. Eliminating the need for an additional, external pump reduces investment costs and saves space in the lab.

Cell retention methods

A number of methods for cell retention are established on the market. Bioreactors come with a spin filter, which is usually mounted on the stirrer shaft within the bioreactor. The cell-free medium is then harvested continuously from the filter interior. Other external systems, such as alternating tangential flow (ATF) or tangential flow filtration (TFF) modules, can also be connected to the bioreactor.

Continuous monitoring for increased productivity

A large number of available sensors, such as viable cell density and biomass sensors, opens up the possibility of continuous monitoring for critical parameters. If cell concentrations are too high, monitoring the biomass concentration online allows researchers to remove cells through a process known as cell bleeding, thereby improving productivity. The use of sensors to measure metabolite concentrations reduces the risk of toxic by-products accumulating within the bioreactor.

Your Perfusion Bioreactor

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  • Exit gas (CO2, O2)
  • Biomass
  • Viable cell density
  • pCO2
  • Redox
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  • Single-use culture vessels
  • Sterile sampling
  • Spin filter
  • Bioprocess software
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Your Perfusion Bioreactor
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Bioreactors for perfusion

Labfors 5

Labfors 5

High-end benchtop bioreactor for any application

  • Total volume up to 13 L

  • Option of additional integrated pumps

  • External balance may be connected

  • Switching between glass and single-use culture vessels (optional)

Multifors 2

Multifors 2

Parallel bioreactor for small-scale bioprocesses

  • Total volume up to 1.4 L

  • Additional integrated pumps (optional)

  • External scales may be connected

  • Up to six fully equipped bioreactors on one HMI

Minifors 2

Minifors 2

The award-winning, all-in-one bioreactor

  • Total volume up to 6 L

  • Additional external pump (optional)

  • External balance may be connected

  • Preconfigured and ready-to-use

eve® –  the bioprocess platform software

eve® – the bioprocess platform software

Workflow-oriented planning tools and comprehensive options for bioprocess control and monitoring

  • Monitor and control bioprocess parameters from any location

  • Powerful feeding strategies

  • Soft sensors for comprehensive process data analysis

  • Validation according to FDA 21 CFR part 11

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We are thoroughly impressed by the practical bioreactor design. It allows us to save time during calibration and preparation for cultivation.
Mohd RazifMamat - Head Of Technical Administration & Operation, Malaysia Genome Institute

«Perfusion processes typically last several weeks, which makes a detailed look inside the culturing process indispensable.»

Lea Düppe — Product Manager

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