Process development with phototrophic organisms
Uniform lighting for cultures plays a crucial role in developing processes with photosynthetically active organisms. In addition to low lighting intensity for initial characterization, this also means reliably generating high intensity light equivalent to sunlight. Precisely measuring and regulating light intensity is crucial for optimized processes with phototrophic organisms.
Scalable platformLarge flat-panel reactors are frequently used for large-scale production of high-quality products with phototrophic organisms under aseptic conditions. To ensure that a process can be readily transferred from the laboratory, one would ideally want to use a similar cultivation system on a laboratory scale. This should likewise include features such as a gentle, low-energy mixing process based on the airlift principle.
Light intensity: the central process parameterThe most important process parameter when culturing photosynthetically active organisms such as algae, plant cells and cyanobacteria is light intensity, which determines the growth rate, cell concentration and thus biomass productivity. Warm white light offers the ideal sunlight-like spectrum within the visible range, and contains a considerable proportion of photosynthetically active light.
Uniform light distribution is indispensableIn addition to light intensity, a successful process also depends on uniform light distribution within the culture. Optimizing the mixing process with respect to light distribution ensures that each cell will be exposed to the light source at brief intervals, and that all of the cells will therefore receive a reliable supply of light.
Simulating natural weather conditionsIf process development is to mimic efficient, solar-powered outdoor production, variable weather conditions need to be taken into account as well. Modern process control accommodates infinitely variable light intensity adjustment and day/night cycle simulation.
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Our solutions for phototrophic process development
Multitron for phototrophic organisms
Sunlight in the shaker with excellent uniformity
Energy-saving, warm-white LED lighting
Light intensity up to 240 μmol m–2s–1
Even distribution of light across the tray
Labfors 5 photobioreactor
Flatbed-photobioreactor for process development
Autoclavable 1.9 L Flatbed culture vessel
LED lighting, intensity up to 3000 µmol m-2s-1
Comprehensive analysis options
eve® – Bioprocess software for bioreactors
Workflow-oriented planning tools and comprehensive options for bioprocess control and monitoring
Location-independent monitoring and control of bioprocess parameters
Detailed reporting according to GMP
Validation according to FDA 21 CFR Part 11