Gene therapy is a promising approach for treating various genetic disorders and diseases. A critical component of gene therapy is the production of viral vectors, which serve as delivery vehicles for therapeutic genes. Human Embryonic Kidney 293 (HEK293) cells have become a widely used platform for viral vector production due to their efficiency in transfection and ability to support viral replication. However, optimizing HEK293 cell cultures for large-scale production of viral vectors remains a challenge in making gene therapies more accessible and cost-effective.
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Gene therapy is a promising approach for treating various genetic disorders and diseases. A critical component of gene therapy is the production of viral vectors, which serve as delivery vehicles for therapeutic genes. Human Embryonic Kidney 293 (HEK293) cells have become a widely used platform for viral vector production due to their efficiency in transfection and ability to support viral replication. However, optimizing HEK293 cell cultures for large-scale production of viral vectors remains a challenge in making gene therapies more accessible and cost-effective.
Simple actions can make a lot of difference to the outcome of your shake flasks cultures. Some beneficial ones have been covered in previous articles, however, common practices can also be a barrier to effective optimization. This article will explain why these common practices count as sins against your shake flask culture and what you can do to overcome them.
In the fast-paced world of bioprocessing, having reliable lab equipment is critical for research and process development success. The Multitron incubator shaker has been designed with these demands in mind, offering precision and efficiency that directly address the challenges scientists face in their labs. Whether you are optimizing culture conditions or scaling up processes, the Multitron shaker provides the tools you need to advance your research with confidence.
As demand for sustainable food sources surges, Daisy Lab is revolutionizing dairy production with precision fermentation technology. By using INFORS HT bioreactors, this New Zealand-based startup is producing animal-free milk proteins like whey and casein. Discover how the innovative process at Daisy Labs, now set to scale with EPA approval, is paving the way for a more sustainable future in dairy.
Cleaning an incubator shaker sounds trivial, but it is critical for cultivation success. The good news is that doing the right thing is simple, effective, and can easily be integrated into the routine management of a laboratory.
Discover how INFORS HT bioreactors and services have supported bioprocess education and research at Universiti Sains Malaysia (USM). Joo Shun TAN (陈裕声), Associate Professor, shares insights on leveraging Minifors, Multifors, and Techfors bioreactors to enhance teaching, research, and scale-up processes.
Discover how INFORS HT’s bioreactor systems enhance bioprocess R&D for the Agro-Biotechnology Institute. Explore their streamlined operations and optimized yields. An interview-style article with Dr. Siti Rokhiyah PhD., Head of Bioreactor Unit at the Agro-Biotechnology Institute (ABI), National Institutes of Biotechnology Malaysia (NIBM) in Malaysia.
Zara Summers, Chief Science Officer at LanzaTech, a Biotech Company, vividly articulates the company goal to reduce fossil fuel consumption and instead, recycle carbon, that is already above ground to create necessities: "We want it so every child in the world can pick up a crayon and color the sky and have that crayon that they pick be blue. Right? And today that is not the reality for every kid around the world."
A plasmid DNA production process was performed comparing conventional Erlenmeyer flasks with LB medium and the Thomson’s Ultra Yield® system. E. coli cells were cultivated in both systems in the INFORS HT Multitron incubator shaker, bacterial growth was monitored, and the plasmids were purified at the end of cultivation. Subsequently, the quality of the isolated plasmids was analyzed by HPLC. The combination of the Ultra Yield® flasks with the enriched Plasmid+® medium and the AirOtop® enhanced seal led to higher cell densities and a 21-fold higher amount of plasmid than in Erlenmeyer shake flasks with LB medium.
In the ever-evolving landscape of biopharmaceutical processes development, companies are constantly seeking ways to enhance efficiency and streamline their operations. One formidable combination that has been gaining momentum is the use of a powerful and easy to use batch planning strategy with eve bioprocessing software and parallel bioreactors Multifors. In this article, we will delve into how the combination of these two tools can enable you to potentiate process development.
Simple actions can make a lot of difference to the outcome of your shake flasks cultures. Some beneficial ones have been covered in previous articles, however, common practices can also be a barrier to effective optimization. This article will explain why these common practices count as sins against your shake flask culture and what you can do to overcome them.
In the fast-paced world of bioprocessing, having reliable lab equipment is critical for research and process development success. The Multitron incubator shaker has been designed with these demands in mind, offering precision and efficiency that directly address the challenges scientists face in their labs. Whether you are optimizing culture conditions or scaling up processes, the Multitron shaker provides the tools you need to advance your research with confidence.
As demand for sustainable food sources surges, Daisy Lab is revolutionizing dairy production with precision fermentation technology. By using INFORS HT bioreactors, this New Zealand-based startup is producing animal-free milk proteins like whey and casein. Discover how the innovative process at Daisy Labs, now set to scale with EPA approval, is paving the way for a more sustainable future in dairy.
Cleaning an incubator shaker sounds trivial, but it is critical for cultivation success. The good news is that doing the right thing is simple, effective, and can easily be integrated into the routine management of a laboratory.
Discover how INFORS HT bioreactors and services have supported bioprocess education and research at Universiti Sains Malaysia (USM). Joo Shun TAN (陈裕声), Associate Professor, shares insights on leveraging Minifors, Multifors, and Techfors bioreactors to enhance teaching, research, and scale-up processes.
Discover how INFORS HT’s bioreactor systems enhance bioprocess R&D for the Agro-Biotechnology Institute. Explore their streamlined operations and optimized yields. An interview-style article with Dr. Siti Rokhiyah PhD., Head of Bioreactor Unit at the Agro-Biotechnology Institute (ABI), National Institutes of Biotechnology Malaysia (NIBM) in Malaysia.
Zara Summers, Chief Science Officer at LanzaTech, a Biotech Company, vividly articulates the company goal to reduce fossil fuel consumption and instead, recycle carbon, that is already above ground to create necessities: "We want it so every child in the world can pick up a crayon and color the sky and have that crayon that they pick be blue. Right? And today that is not the reality for every kid around the world."
A plasmid DNA production process was performed comparing conventional Erlenmeyer flasks with LB medium and the Thomson’s Ultra Yield® system. E. coli cells were cultivated in both systems in the INFORS HT Multitron incubator shaker, bacterial growth was monitored, and the plasmids were purified at the end of cultivation. Subsequently, the quality of the isolated plasmids was analyzed by HPLC. The combination of the Ultra Yield® flasks with the enriched Plasmid+® medium and the AirOtop® enhanced seal led to higher cell densities and a 21-fold higher amount of plasmid than in Erlenmeyer shake flasks with LB medium.
In the ever-evolving landscape of biopharmaceutical processes development, companies are constantly seeking ways to enhance efficiency and streamline their operations. One formidable combination that has been gaining momentum is the use of a powerful and easy to use batch planning strategy with eve bioprocessing software and parallel bioreactors Multifors. In this article, we will delve into how the combination of these two tools can enable you to potentiate process development.