Automated feeding of liquids in shake flasks

LIS - Liquid Injection System

LIS is the first easy-to-use technology allowing for automatic, parallelized and uncomplicated feeding of liquids in shake flasks. The systems consists of three components: the drive, the cartridge and optionally the software. Various predefined feeding profiles allow users to quickly create individual feeding strategies for their shake flask cultures. By using the LIS software, experiments can be monitored and controlled wirelessly. Additionally, it allows you to configure indivisual feeding profiles. LIS is compatible with various substances such as sugars, alcohols as well as acids or bases.

LIS - Liquid Injection System

For fed-batch experiments in shake flask cultivations
Suitable for bacteria, algae and cell cultures
Compatible with all types of laboratory shakers and various substances
Customizable feeding profiles

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LIS - Liquid Injection System
For fed-batch experiments in shake flask cultivations

Compatible with all types of laboratory shakers

LIS is compatible with all types of laboratory shakers, spring clamps and Sticky Stuff, as well as all 38 mm straight neck glass and plastic flasks.

Wireless montoring and control

Control and monitor your feeding process via the LIS software and the bioprocess platform eve®.

Can be used for a diverse range of scientific applications

e.g. Fed-batch experiments, passive pH control, automatic induction of protein expression, toxicity tests, phage assays, co-cultures

Features

Cartridge The cartridge is a sterile single us container with the shape of a lid. It fits on all shake flasks that are normally sealed with a metal cap and can be filled with up to 25 mL of the feeding liquid.
Drive The drive is a programmable miniature pump that controls when and how much liquid is fed from the cartridge into the flask. You can either program the feeding profile in the drive manually using the knob on top of the drive or wirelessly by using the LIS software.
eve® platform software for your bioprocesses With eve® the LIS pump can be controlled remotely and complex feeding strategies such as exponential feeding or time/event-based feeding strategies can easily be configured. The values are monitored along other bioprocess parameters and saved in a central database.
Drive Input voltage: 5 CDC +/- 5 % Input current: min. 200 mA
Cartridge Sterilization: Ethylene Oxide Fitting flask type: straight neck (no brim) Fitting flask outside diameter: 38 mm (+/- 0,3 mm); fitting flask minimum inside diameter: 33 mm
Pump flow rate max. 1 ml/min
Battery
  • Type: Li-Ion
  • Capacity: 600 mAh
  • Run time: ca. 1 week depending on feeding profile
  • Charging time: ca 2 hours
  • Charging source: USB 2.0 Port
  • Dimensions height (with mounting ring) ca. 88 mm; additional height on shake flask: ca. 70 mm
    Rotation speed 200 min-1 - 300 min-1 depending on shaking throw and shake flask size
    Shake flask sizes 100 ml
    250 ml
    300 ml
    500 ml
    1000 ml
    2000 ml
    Temperature 10 – 45 °C
    Ambient humidity (rH) Relative humidity: 0 – 80% (not-condensing)
    System requirements CPU: min. dual core 2 CHz
  • RAM / HDD: ≥4 GB / ≥10 GB
  • Operating system: Microsoft Windows 7 or newer with JRE 8 and .NET 4.5
  • Screen resolution: 1920 x 1080 pixels
  • Recommended Web browser: Firefox, Chrome
  • Manuals

    LIS User Guide (1.7 MB)

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    White Papers

    Automated Liquid Addition in Shake Flasks (1.1 MB)

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    LIS - Liquid Injection System
    Drive Input voltage: 5 CDC +/- 5 % Input current: min. 200 mA
    Cartridge Sterilization: Ethylene Oxide Fitting flask type: straight neck (no brim) Fitting flask outside diameter: 38 mm (+/- 0,3 mm); fitting flask minimum inside diameter: 33 mm
    Pump flow rate max. 1 ml/min
    Battery
  • Type: Li-Ion
  • Capacity: 600 mAh
  • Run time: ca. 1 week depending on feeding profile
  • Charging time: ca 2 hours
  • Charging source: USB 2.0 Port
  • Dimensions height (with mounting ring) ca. 88 mm; additional height on shake flask: ca. 70 mm
    Rotation speed 200 min-1 - 300 min-1 depending on shaking throw and shake flask size
    Shake flask sizes 100 ml
    250 ml
    300 ml
    500 ml
    1000 ml
    2000 ml
    Temperature 10 – 45 °C
    Ambient humidity (rH) Relative humidity: 0 – 80% (not-condensing)
    System requirements CPU: min. dual core 2 CHz
  • RAM / HDD: ≥4 GB / ≥10 GB
  • Operating system: Microsoft Windows 7 or newer with JRE 8 and .NET 4.5
  • Screen resolution: 1920 x 1080 pixels
  • Recommended Web browser: Firefox, Chrome
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    «LIS ist einfach zu bedienen, ermöglicht höhere Biomassekonzentrationen und verhindert Kultivierungsunterbrüche, die durch manuelle Fütterung der Kultur notwendig wären.»

    Lea Düppe — Produktmanagerin

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