High cell densities in bench-top bioreactors

Labfors 5 for microorganisms

The Labfors 5 is ideally suited for cultivation of bacteria and yeasts. The bioreactor has a powerful motor, options for high oxygenation and a good cooling system. It can be equipped, if needed, with additional sensors or scales so that any feeding strategy can be carried out and very high cell counts can be easily achieved.

Labfors 5 for microorganisms

Perfect cultivation conditions for bacteria and yeasts
Ideal for E. coli and Pichia pastoris
High performance stirring system with up to 1500 min–1
Optimum gassing with up to 5 gas lines
eve® Bioprocess Platform Software

Optional

Volume (TV)
Gassing
  • Air
  • O2
  • N2
  • CO2
Sensors
  • Biomass
  • Exit gas (CO2>, O2>)
  • Redox
I am interested in:
  • CIP/SIP
  • Bioprocess software
  • Gravimetric feeding
  • Additional substrate pump
  • Qualification

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Labfors 5 for microorganisms
Perfect cultivation conditions for bacteria and yeasts

Optimal oxygenation for maximum growth rates

High-cell-density cultures (HCDCs) have an increased need for oxygen during the growth phase. For this reason, the Labfors 5 has a powerful motor for perfect mixing and individual control of the gas supply with up to 5 MFCs. The increasing need for oxygen can be met at all times through the combination of stirring speed and gassing.

Intelligent feeding strategies

Controlling the substrate concentration plays a central role in high-cell-density processes. You control the pump for the addition of substrate either directly at the bioreactor or using the eve® bioprocess software. You may of course save and reuse your individual feeding profiles. Connecting a scale also allows gravimetric feeding for an even more precise addition of nutrients over long periods of time.

Optimized metabolic processes & product yield

Using the eve® bioprocess software, you can specifically analyze and optimize the metabolic processes in your culture. For example, you can perform exponential feeding using the respiratory quotient (RQ).

Features

Culture vessels
  • Working volumes ranging between 0.5 and 10 L
  • Can be configured according to individual needs thanks to a selection of different stirrers, spargers and specialized accessories
  • Up to 14 ports for sensors (antifoam, optical density, pH, pO2, redox, conductivity, live cell density, etc.)
  • No welded lid components—ideal for GMP applications
  • High-performance pumps
  • Basic setup includes four precision pumps per culture vessel (option of one more)
  • Autoclavable pump heads
  • Gravimetric feeding (with external scale)
  • Profile-based pump-speed control with eve®
  • Gassing
  • Customer-specific gassing with up to 5 gas lines, either via a sparger or in the headspace
  • Feed via rotameter or mass-flow regulator
  • Cascades may be configured in serial or in parallel for controlling the pO2 via the stirrer speed, gassing rate, gas composition, etc.
  • pH control with acid and CO2
  • Sensors
  • Customers may choose from digital (Modbus) or analog sensors.
  • Product calibration of the pH sensor for reliable measurements during long-running bioprocesses
  • If required, additional sensors and actuators can be connected.
  • Cleaning and sterilisation
  • Automatic cleaning (CIP) and sterilisation (SIP) of all parts in contact with the product
  • Double throughput possible due to overnight cleaning
  • Reliable, reproducible and validatable base and/or acid cleaning
  • available as an option
  • LabCIP is patented (US Pat. No. 9,399,244; European patent No. 2 789 683).
  • Operating unit
  • Touchscreen with integrated OPC server
  • Reliable measurement and setting of the bioprocess parameters and communication with eve®
  • Parallel management and control of up to six devices
  • Up to 24 selectable parameters, such as temperature, stirring speed, pH, pO2, anti-foaming agent, feed
  • eve® Bioprocess Platform Software
  • Planning, controlling and analysing bioprocesses
  • Integrated workflows, devices and bioprocess knowledge
  • Integrates bioreactors and analytical instruments, regardless of the manufacturer
  • Web-based project organisation
  • Communication using the latest OPC UA (Minifors 2) or OPC XML-DA standard (Multifors 2, Labfors 5, Techfors-S, Techfors)
  • Synchronisation of process-related events such as sampling or inoculation
  • Vessels 2 L / 3,6 L / 7,5 L / 13 L
    Working volume 0,5–1,2 L / 0,5–2,3 L / 1–5 L / 2,2–10 L
    Dimensions (W x D x H) 464 mm x 462 mm x 996 mm
    Drive direct drive up to 1500 min–1
    Temperature coolant temperature + 5 °C bis 70 °C bzw. 95 °C
    Gassing per vessel up to 5 rotameters or MFCs up to 2 min-1 (vvm)
    Pumps per vessel 3 fixed, 1 variable, optional 2 additional variable
    Ports per vessel 7 mm max. 4x 10 mm 2x 12 mm (Pg13,5) max. 6x 19 mm max 6x
    Connectivity OPC XML DA via Ethernet
    Parallel operation via touch screen controller up to 6 vessels
    Sterilisation Autoclave or LabCIP
    Labfors 5 for microorganisms
    Vessels 2 L / 3,6 L / 7,5 L / 13 L
    Working volume 0,5–1,2 L / 0,5–2,3 L / 1–5 L / 2,2–10 L
    Dimensions (W x D x H) 464 mm x 462 mm x 996 mm
    Drive direct drive up to 1500 min–1
    Temperature coolant temperature + 5 °C bis 70 °C bzw. 95 °C
    Gassing per vessel up to 5 rotameters or MFCs up to 2 min-1 (vvm)
    Pumps per vessel 3 fixed, 1 variable, optional 2 additional variable
    Ports per vessel 7 mm max. 4x 10 mm 2x 12 mm (Pg13,5) max. 6x 19 mm max 6x
    Connectivity OPC XML DA via Ethernet
    Parallel operation via touch screen controller up to 6 vessels
    Sterilisation Autoclave or LabCIP
    The Labfors bioreactors, with their modular philosophy, give us enormous flexibility.
    Prof. Dr. Christoph Herwig - Head of Biochemical Engineering, Vienna Technical University

    «Der beste Bioreaktor für anspruchsvolle Kultivierungen mit hoher Zelldichte.»

    Lea Düppe — Product Manager Bioprocess Applications

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