Gut Simulation in the Bioreactor
The gut, especially its microbiome, is a highly complex system that has, in the past, proven difficult to precisely replicate in vitro. Therefore, in vivo models were often used which posed several challenges including limited reproducibility, repeatability and stability. However, recent advances have enabled researchers to mimic this ecosystem using specially adapted bioreactors which are able to provide an environment representative of the microbial communities that populate the gut.
How to mimic the ecosystem of the gut
Essentially, the environment within the bioreactor will need to be free from oxygen, with a minimum flow of N2 and appropriate levels of mixing and control. Low stirrer speeds are necessary to mimic the gut environment. Depending on the aim of the research, gassing with CO2 and monitoring the redox potential may be required.
Characterisation of the influence of external stimulus
The bioreactor can be programmed for continuous chemostat operation. Once the bioreactor has reached equilibrium or steady state, many researchers choose to perform perturbation experiments to characterise how the ecosystem from the gut reacts to external stimuli such as different feed substrates or other bacterial species, or even to validate the functionality of new ecosystem therapeutic drugs.
Reliable bioreactor for long-lasting microbiome studiesThe human gut microbial ecosystem is extremely diverse and complex. Therefore, a bioreactor that can simplify the day-to-day operational needs of the ecosystem is needed. Moreover, it is important that the device is reliable and of good quality, since the experiments can take several weeks to complete, and they need to be reproducible.
Parallel experiments for more results in less timeHaving the option to run several bioreactors at the same time makes experimental design user-friendly, simple and efficient. It is also advantageous for all the culture vessels to be controlled and monitored from one touchscreen, reducing repetition of the daily operational tasks and the likelihood of manual errors.
Application for parallel bioprocesses
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Your solution for gut simulation
Parallel bioreactor system for small-scale bioprocesses - compact design for up to 6 fully equipped bioreactors
Ideal mixing at low stirrer speeds
Keeps anaerobic conditions with low N2 flow
Compact design for up to 6 fully equipped bioreactors
Additional feed pump for continuous mode available
Workflow-oriented planning tools and comprehensive options for bioprocess control and monitoring
Location-independent monitoring and control of bioprocess parameters
Detailed reporting according to GMP
Validation according to FDA 21 CFR Part 11