Non-invasive online monitoring of biomass in shake flasks

CGQ - Cell Growth Quantifier

The Cell Growth Quantifier (CGQ) is a laboratory device for online monitoring of biomass in shake flasks without taking the flasks out of the incubation shaker. The CGQ determines the biomass concentration automatically and non-invasively by a patented optical measurement. Thus, profound microbial growth kinetics of highest precision can be generated.

CGQ - Cell Growth Quantifier

The new standard for biomass monitoring
Parallel applications
Suitable for algae, Archaea, bacteria, fungi, plant cells, yeast
Fits ideally on all glass and single-use flasks

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Flask size
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CGQ - Cell Growth Quantifier
The new standard for biomass monitoring

Make sense of your bioprocess

Creating a seamless record of biomass concentration allows you to detect bioprocess changes in real time. This lets you identify limitations, inhibiting factors, and contamination a t an early stage. Reproducibility is increased due to a lower error rate when compared with manual biomass measurement.

Parallel applications

Monitor up to 64 shake flasks in parallel one one PC

Real-time measurement

Detailed process monitoring at any given time for a high data density

Can be used for a diverse range of scientific applications

Growth-curve-guided induction of protein expression, media development / optimisation, screening / strain comparisons, detection of limitations and contaminations, analysis of growth kinetics, optimisation of culture conditions, online monitoring of thermophile and phototrophic organisms

Features

Sensor plate The sensor plate is positioned under the shake flask and measures the biomass non-invasively. It is available for 5 flask sizes.
Cover Each shake flask can be darkened with a cover to ensure measurements of highest precision and sensitivity.
Base station The base station designed for 8 or 16 shake flasks bundles the data from all monitored flasks and sends it to the cgQuant software.
CGQuant Software The cgQuant Software analyses and visualises the biomass signal from all monitored shake flasks.
Compatibility With the INFORS HT shakers and standard laboratory equipment of all manufacturers. Fits ideally on all glass and single-use flasks (with and without baffles), spring clamps and sticky plates («Sticky Stuff»).
Shake flask sizes 100 mL
250 mL
500 mL
1000 mL
2000 mL
5000 mL
Rotation speed 150 - 350 min-1 (clamps)
0 - 200 min-1 (StickyStuff)
Temperature 10 - 50 °C
Ambient humidity (rH) 0 - 80% (non-condensing)
Measuring range OD600 0,2 to 5013
System requirements Windows 7 or newer with .NET 4.7; Dual Core @ 2 GHz/core; >8 GB RAM; >10 GB HDD

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Manuals

Operating manual CGQ (3.8 MB)

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CGQ - Cell Growth Quantifier
Shake flask sizes 100 mL
250 mL
500 mL
1000 mL
2000 mL
5000 mL
Rotation speed 150 - 350 min-1 (clamps)
0 - 200 min-1 (StickyStuff)
Temperature 10 - 50 °C
Ambient humidity (rH) 0 - 80% (non-condensing)
Measuring range OD600 0,2 to 5013
System requirements Windows 7 or newer with .NET 4.7; Dual Core @ 2 GHz/core; >8 GB RAM; >10 GB HDD
The CGQ has enabled us to obtain accurate information on the growth kinetics of hyperthermophilic archaea and has solved the problem of manually generating data for prolonged periods of time and of dealing with evaporation effects due to the high temperature.
Prof. Dr. ir. Eveline Peeters - Group Leader at Vrije Universiteit Brussel, Belgium

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