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CHO (Chinese Hamster Ovary) cells are the most commonly used resource for complex recombinant proteins for therapeutic use. Their importance for bioprocessing is already significant and growing.
The impeller, also known as agitator, is a key component of the bioreactor. It performs the important tasks of mixing, aeration, heat and mass transfer within the vessel. Learn about the different characteristics of various types of impeller and the importance of their designs.
In order to understand the optimum conditions for in-vitro culture, you need to know which natural environment gives the organism what they need to grow and thrive. In addition, a clear understanding of the different growth phases in a bioprocess is essential for selecting a bioprocess strategy.
Setting up a bioreactor is not as easy as it sometimes sounds. Which sensors do I have to calibrate before autoclaving? Is it important to protect the filters with foil from water condensate in the autoclave? These are only some questions which arise when preparing a bioreactor for its next cultivation run. This blog post will guide you through the assembly of a bioreactor step by step and provide practical help for successfully starting a bioprocess.
For a successful aerobic cultivation, a good oxygen transfer is vital. The determination of the volumetric oxygen transfer coefficient (kLa) and the OTR helps you to optimize the bioprocess in bioreactors and ensure a successful scale-up process.
Decisive bioprocess parameters such as biomass and product concentration or metabolite concentrations in the medium are often not directly measurable, requiring offline sampling which does not provide immediate results. Despite the development of new measuring systems and probes, not every parameter can always be measured directly online. This can be remedied by soft-sensors (sometimes also called software sensors), which calculate the desired parameter based on a model or a simple correlation of known measured values.
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