Author Archives: Tony Allman

Monoclonal antibodies have been used for testing kits and medical therapies across a wide range of applications. The most recent is to provide a potential treatment for patients suffering from Covid-19 infection. The production of monoclonal antibodies is well understood, scalable and standardized to the extent that it makes a good generic example of a modern bioprocess. This aspect will be the focus of this short overview. 

Buying a new incubator shaker may seem like a simple job. You could select the same model again, the cheapest version, or the shaker with the fastest delivery time. Nothing to worry about as it’s a simple standard product, so anything will do the job. However, this approach leaves out a vital factor – what quality of results can you expect at the other end of your bioprocess? 

The shake flask is one of the standard laboratory tools for culture of microorganisms and cells. The bioreactor, conversely, is seen as a specialized, solution for commercial applications and academic departments such as chemical engineering. While there is a generic case to be made for this view, the realities of modern research in the biosciences have expanded the need for more information and semi-production capability at small scales.

This article will look at the history and development of a de-facto standard in academic and industrial research laboratories throughput the world, the Multitron incubator shaker. The aim is to show that an initial revolution can lead to evolution, as needs change over time. The fundamentals of a good design can last decades, when new benefits and the best of current technologies are added regularly.

CHO (Chinese Hamster Ovary) cells are the most commonly used resource for complex recombinant proteins for therapeutic use. Their importance for bioprocessing is already significant and growing.  

In order to understand the optimum conditions for in-vitro culture, you need to know which natural environment gives the organism what they need to grow and thrive. In addition, a clear understanding of the different growth phases in a bioprocess is essential for selecting a bioprocess strategy.