Pichia pastoris : High cell density culture in the bioreactor

Minifors 2 for Pichia pastoris

The Minifors 2 bioreactor comes with all of the functions and features you need for cultivating P. pastoris successfully. Even when working with highly viscous P. pastoris cultures, the powerful motor mixes the culture thoroughly and ensures an adequate supply of oxygen. In order to counteract the considerable heat that develops during yeast metabolism, the Minifors 2 comes with a high-performance cooling system and, if necessary, an additional cold finger. Integrated pumps allow for precise glucose and methanol feeding.

Minifors 2 for Pichia pastoris

A complete bioreactor package

High-performance stirring system capable of up to 1600 min^-1

4 configurable pumps

pO₂ regulation with two MFCs (air/O₂) for high cell densities

Powerful cooling system with additional cold finger

Also included for beginners: a “cookbook” explaining bioprocess fundamentals, incl. formulations.

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Minifors 2 for Pichia pastoris
A complete bioreactor package

High cell densities with Pichia pastoris — the Minifors 2 makes it easy

The high-performance motor of the Minifors 2 optimizes oxygen delivery, even when working with high-density Pichia pastoris culture. Oxygen supply can be improved even further with the integrated GasMix system, which consists of two mass-flow controllers (MFCs) that enable precise, infinitely variable pO₂ regulation. The Minifors 2 has an efficient temperature control system that can be enhanced through the use of a cold finger. This maintains the optimum temperature range - even for highly exothermic Pichia pastoris cultures.

Analysis options to help you understand bioreactor processes better

Online exit-gas and biomass-analysis sensors (O₂, CO₂, turbidity) can be installed directly within the system, allowing you to monitor the culture status of P. pastoris in real time. In addition, external balances also enable precise, gravimetric feeding for glucose and methanol.

eve^® Bioprocess Platform Software

eve^® is able to do more than just plan, control and analyse your bioprocesses. The eve^® software integrates workflows, devices, bioprocess information and big data in a web-based platform, with which you can organise your bioprocesses in a web based system.

Features

Culture vessels	Available in total volumes of 1.5 L, 3 L and 6 L Quick and easy change between culture vessel sizes Rounded flat vessel bottom: Low minimum working volume, good mixing, stability with or without a vessel holder Safe sampling without any dead volume: INFORS HT Super Safe Sampler
Top plate	Intelligent design for easy access to all ports Sufficient ports, for standard fittings and extra inlets, immersion tubes and sensors 4 x 7.5 mm: addition ports available for acid, base, feed & 1 free 4 x 10 mm: sparger, anti-foam system, temperature sensor, sampling/harvest Maximum 7 x 12 mm (Pg13.5): pH, pO₂, exhaust gas cooler, inoculation are pre-allocated, 3 are freely available Convenient, tool-free cleaning and maintenance thanks to knurled screws
High-performance pumps	4 High-performance pumps Configurable operating mode: digital (fixed-speed) or analogue (variable speed) 3 x fix (acid, base, antifoam), 1 x variable (feed) Autoclavable pump heads Gravimetric feeding with dosing mode (requires external scale) Profile-based pump-speed control (with eve^®) 2 analogue inputs / outputs to connect external pumps (version for cell cultures)
Gassing	2 integrated MFC for air, air/O₂, or air/N₂ (version for microorganisms) 5 integrated MFC for air, O₂, N₂, CO₂ and head space gassing (version for cell cultures) Integrated pressure sensor for detecting a blocked filter Gassing optimised for bacteria applications and cell cultures: specific gassing rate (vvm) up to 2.0 min^-1 Multiple cascades for pO₂ control by means of stirrer speed, gassing rate (“TotalFlow”) and/or gas composition (“GasMix”)
Sensors	Robust, digital connection of pH and pO₂ sensors Optical pO₂ sensor: immediately ready for use, no polarization required Calibration data stored in sensor head Product calibration of the pH sensor for reliable measurements during long-running bioprocesses Optional: biomass sensors from Optek and aquila biolabs
Operating unit	Integrated 7" touch screen (17.8 cm) Simple handling through intuitive menus and practical on-screen help Calibration of pH, pO₂ and OD sensors using simple install wizards Direct data export to USB drive in stand-alone operation Multiple display languages
eve^® Bioprocess Platform Software	Planning, controlling and analysing bioprocesses Integrated workflows, devices and bioprocess knowledge Integrates bioreactors and analytical instruments, regardless of the manufacturer Web-based project organisation Communication using the latest OPC UA (Minifors 2) or OPC XML-DA standard (Multifors 2, Labfors 5, Techfors-S, Techfors) Synchronisation of process-related events such as sampling or inoculation

Technical Details

Vessels	1,5 L / 3 L / 6 L
Working volume	0,3–1,0 L / 0,6–2,0 L / 1,1–4,0 L
Dimensions (W x D x H)	455 mm x 375 mm x 740 mm
Drive	Version for Microorganisms: 150 min^-1 to 1600 min^-1 Version for cell cultures: 24 min–1 to 600 min^-1
Temperature	Coolant temperature + 10 °C bis 60 °C
Gassing per vessel	Version for microorganisms: 2 MFCs up to 2 min^-1 Version for cell cultures: 5 MFCs up to 0.15 min^-1
Pumps per vessel	4 x configurable (fixed or variable speed) ex-works: 3 x fixed (acid, base, anti-foam), 1 x variable (feed)
Pump performance	0,0034 to 3,52 mL min^-1 (standard), 0,017 to 16,13 mL min^-1, 0,0012 to 1,12 mL min^-1
Ports per vessel	7,5 mm 4x 10 mm 4x 12 mm (Pg13,5) max. 7x
Connectivity	OPC UA via Ethernet
Parallel operation via touch screen controller	n/a
Sterilisation	Autoclave

Downloads

Flyer & Brochures

Flyer Minifors 2 (725.4 kB)

INFORS HT - Our Products (2.1 MB)

Practical Advice

The Cookbook - A Guide for Biotech Beginners (2.0 MB)

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Application Notes

Biological and process-engineering characterization of the Minifors 2 laboratory bioreactor (429.9 kB)

Manuals

Operating manual Minifors 2 (5.9 MB)

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Compare

	Minifors 2 for Pichia pastoris
Vessels	1,5 L / 3 L / 6 L
Working volume	0,3–1,0 L / 0,6–2,0 L / 1,1–4,0 L
Dimensions (W x D x H)	455 mm x 375 mm x 740 mm
Drive	Version for Microorganisms: 150 min^-1 to 1600 min^-1 Version for cell cultures: 24 min–1 to 600 min^-1
Temperature	Coolant temperature + 10 °C bis 60 °C
Gassing per vessel	Version for microorganisms: 2 MFCs up to 2 min^-1 Version for cell cultures: 5 MFCs up to 0.15 min^-1
Pumps per vessel	4 x configurable (fixed or variable speed) ex-works: 3 x fixed (acid, base, anti-foam), 1 x variable (feed)
Pump performance	0,0034 to 3,52 mL min^-1 (standard), 0,017 to 16,13 mL min^-1, 0,0012 to 1,12 mL min^-1
Ports per vessel	7,5 mm 4x 10 mm 4x 12 mm (Pg13,5) max. 7x
Connectivity	OPC UA via Ethernet
Parallel operation via touch screen controller	n/a
Sterilisation	Autoclave