Pichia pastoris : High cell density culture in the bioreactor

Minifors bioreactor Pichia pastoris Minifors 2 bioreactor Pichia pastoris

Minifors 2 for Pichia pastoris

The Minifors 2 bioreactor comes with all of the functions and features you need for cultivating P. pastoris successfully. Even when working with highly viscous P. pastoris cultures, the powerful motor mixes the culture thoroughly and ensures an adequate supply of oxygen. In order to counteract the considerable heat that develops during yeast metabolism, the Minifors 2 comes with a high-performance cooling system and, if necessary, an additional cold finger. Integrated pumps allow for precise glucose and methanol feeding.

Minifors 2 for Pichia pastoris

A complete bioreactor package
High-performance stirring system capable of up to 1600 min-1
4 configurable pumps
pO2 regulation with two MFCs (air/O2) for high cell densities
Powerful cooling system with additional cold finger
Also included for beginners: a “cookbook” explaining bioprocess fundamentals, incl. formulations.

Optional

Volume (TV)
I am interested in:
  • Exit gas analysis
  • Bioprocess software
  • Gravimetric feeding
  • Online biomass sensors
  • Additional cold finger

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Minifors 2 for Pichia pastoris
A complete bioreactor package

High cell densities with Pichia pastoris — the Minifors 2 makes it easy

The high-performance motor of the Minifors 2 optimizes oxygen delivery, even when working with high-density Pichia pastoris culture. Oxygen supply can be improved even further with the integrated GasMix system, which consists of two mass-flow controllers (MFCs) that enable precise, infinitely variable pO2 regulation. The Minifors 2 has an efficient temperature control system that can be enhanced through the use of a cold finger. This maintains the optimum temperature range - even for highly exothermic Pichia pastoris cultures.

Analysis options to help you understand bioreactor processes better

Online exit-gas and biomass-analysis sensors (O2, CO2, turbidity) can be installed directly within the system, allowing you to monitor the culture status of P. pastoris in real time. In addition, external balances also enable precise, gravimetric feeding for glucose and methanol.

eve® Bioprocess Platform Software

eve® is able to do more than just plan, control and analyse your bioprocesses. The eve® software integrates workflows, devices, bioprocess information and big data in a web-based platform, with which you can organise your bioprocesses in a web based system.

Features

Culture vessels
  • Available in total volumes of 1.5 L, 3 L and 6 L
  • Quick and easy change between culture vessel sizes
  • Rounded flat vessel bottom: Low minimum working volume, good mixing, stability with or without a vessel holder
  • Safe sampling without any dead volume: INFORS HT Super Safe Sampler
  • Top plate
  • Intelligent design for easy access to all ports
  • Sufficient ports, for standard fittings and extra inlets, immersion tubes and sensors
  • 4 x 7.5 mm: addition ports available for acid, base, feed & 1 free
  • 4 x 10 mm: sparger, anti-foam system, temperature sensor, sampling/harvest
  • Maximum 7 x 12 mm (Pg13.5): pH, pO2, exhaust gas cooler, inoculation are pre-allocated, 3 are freely available
  • Convenient, tool-free cleaning and maintenance thanks to knurled screws
  • High-performance pumps
  • 4 High-performance pumps
  • Configurable operating mode: digital (fixed-speed) or analogue (variable speed)
  • 3 x fix (acid, base, antifoam), 1 x variable (feed)
  • Autoclavable pump heads
  • Gravimetric feeding with dosing mode (requires external scale)
  • Profile-based pump-speed control (with eve®)
  • 2 analogue inputs / outputs to connect external pumps (version for cell cultures)
  • Gassing
  • 2 integrated MFC for air, air/O2, or air/N2 (version for microorganisms)
  • 5 integrated MFC for air, O2, N2, CO2 and head space gassing (version for cell cultures)
  • Integrated pressure sensor for detecting a blocked filter
  • Gassing optimised for bacteria applications and cell cultures: specific gassing rate (vvm) up to 2.0 min-1
  • Multiple cascades for pO2 control by means of stirrer speed, gassing rate (“TotalFlow”) and/or gas composition (“GasMix”)
  • Sensors
  • Robust, digital connection of pH and pO2 sensors
  • Optical pO2 sensor: immediately ready for use, no polarization required
  • Calibration data stored in sensor head
  • Product calibration of the pH sensor for reliable measurements during long-running bioprocesses
  • Optional: biomass sensors from Optek and aquila biolabs
  • Operating unit
  • Integrated 7" touch screen (17.8 cm)
  • Simple handling through intuitive menus and practical on-screen help
  • Calibration of pH, pO2 and OD sensors using simple install wizards
  • Direct data export to USB drive in stand-alone operation
  • Multiple display languages
  • eve® Bioprocess Platform Software
  • Planning, controlling and analysing bioprocesses
  • Integrated workflows, devices and bioprocess knowledge
  • Integrates bioreactors and analytical instruments, regardless of the manufacturer
  • Web-based project organisation
  • Communication using the latest OPC UA (Minifors 2) or OPC XML-DA standard (Multifors 2, Labfors 5, Techfors-S, Techfors)
  • Synchronisation of process-related events such as sampling or inoculation
  • Vessels 1,5 L / 3 L / 6 L
    Working volume 0,3–1,0 L / 0,6–2,0 L / 1,1–4,0 L
    Dimensions (W x D x H) 455 mm x 375 mm x 740 mm
    Drive Version for Microorganisms: 150 min-1 to 1600 min-1 Version for cell cultures: 24 min–1 to 600 min-1
    Temperature Coolant temperature + 10 °C bis 60 °C
    Gassing per vessel Version for microorganisms: 2 MFCs up to 2 min-1 Version for cell cultures: 5 MFCs up to 0.15 min-1
    Pumps per vessel 4 x configurable (fixed or variable speed) ex-works: 3 x fixed (acid, base, anti-foam), 1 x variable (feed)
    Pump performance 0,0034 to 3,52 mL min-1 (standard), 0,017 to 16,13 mL min-1, 0,0012 to 1,12 mL min-1
    Ports per vessel

    7,5 mm                 4x
    10 mm                  4x
    12 mm (Pg13,5)   max. 7x

    Connectivity OPC UA via Ethernet
    Parallel operation via touch screen controller n/a
    Sterilisation Autoclave

    Flyer & Brochures

    Practical Advice

    The Cookbook - A Guide for Biotech Beginners (2.0 MB)

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    Application Notes

    Manuals

    Operating manual Minifors 2 (8.3 MB)

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    Minifors 2 for Pichia pastoris
    Vessels 1,5 L / 3 L / 6 L
    Working volume 0,3–1,0 L / 0,6–2,0 L / 1,1–4,0 L
    Dimensions (W x D x H) 455 mm x 375 mm x 740 mm
    Drive Version for Microorganisms: 150 min-1 to 1600 min-1 Version for cell cultures: 24 min–1 to 600 min-1
    Temperature Coolant temperature + 10 °C bis 60 °C
    Gassing per vessel Version for microorganisms: 2 MFCs up to 2 min-1 Version for cell cultures: 5 MFCs up to 0.15 min-1
    Pumps per vessel 4 x configurable (fixed or variable speed) ex-works: 3 x fixed (acid, base, anti-foam), 1 x variable (feed)
    Pump performance 0,0034 to 3,52 mL min-1 (standard), 0,017 to 16,13 mL min-1, 0,0012 to 1,12 mL min-1
    Ports per vessel

    7,5 mm                 4x
    10 mm                  4x
    12 mm (Pg13,5)   max. 7x

    Connectivity OPC UA via Ethernet
    Parallel operation via touch screen controller n/a
    Sterilisation Autoclave
    We are thoroughly impressed by the practical bioreactor design. It allows us to save time during calibration and preparation for cultivation.
    Mohd RazifMamat - Head Of Technical Administration & Operation, Malaysia Genome Institute

    «As a consequence of user-centered development, we have created a bioreactor that is unbeatable for its ease of use.»

    Lea Düppe — Product Manager

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